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cat 79774 61563 75015  (Addgene inc)


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    Structured Review

    Addgene inc cat 79774 61563 75015
    Cat 79774 61563 75015, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+sfgfp/pET28a-ybbR-HIS-sfGFP-DocI+(Plasmid+%2358708)/pm41714452-197-12-11
    Average 90 stars, based on 2 article reviews
    cat 79774 61563 75015 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Sortase mediated protein ubiquitination with defined chain length and topology
    Article Snippet: .. C-terminal His-tag was added to these constructs using Q5 Site-Directed Mutagenesis. pet30b-5M SrtA and pet30b-7M SrtA plasmids were a gift from Hidde Ploegh (Addgene plasmids # 51140 and # 51141). pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492) and V1G mutation and N150TAG mutations were added using Q5 Site-Directed Mutagenesis. pDULE-CNF was a gift from Ryan Mehl (Addgene plasmid # 85494). pET28-UBE1 was a gift from Deschaies lab. pDEST17-Cdc34a was a gift from Wade Harper (Addgene plasmid # 18674). pET28aLIC-UEV1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25619). pETSUMOhUbc13 was a gift from Cynthia Wolberger (Addgene plasmid # 51131). pET16b-PCNA was a gift from Andrew Deans (Addgene plasmid # 134898). ..

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Mutagenesis:

    Article Title: Sortase mediated protein ubiquitination with defined chain length and topology
    Article Snippet: .. C-terminal His-tag was added to these constructs using Q5 Site-Directed Mutagenesis. pet30b-5M SrtA and pet30b-7M SrtA plasmids were a gift from Hidde Ploegh (Addgene plasmids # 51140 and # 51141). pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492) and V1G mutation and N150TAG mutations were added using Q5 Site-Directed Mutagenesis. pDULE-CNF was a gift from Ryan Mehl (Addgene plasmid # 85494). pET28-UBE1 was a gift from Deschaies lab. pDEST17-Cdc34a was a gift from Wade Harper (Addgene plasmid # 18674). pET28aLIC-UEV1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25619). pETSUMOhUbc13 was a gift from Cynthia Wolberger (Addgene plasmid # 51131). pET16b-PCNA was a gift from Andrew Deans (Addgene plasmid # 134898). ..

    Plasmid Preparation:

    Article Title: Sortase mediated protein ubiquitination with defined chain length and topology
    Article Snippet: .. C-terminal His-tag was added to these constructs using Q5 Site-Directed Mutagenesis. pet30b-5M SrtA and pet30b-7M SrtA plasmids were a gift from Hidde Ploegh (Addgene plasmids # 51140 and # 51141). pET28a-sfGFP was a gift from Ryan Mehl (Addgene plasmid # 85492) and V1G mutation and N150TAG mutations were added using Q5 Site-Directed Mutagenesis. pDULE-CNF was a gift from Ryan Mehl (Addgene plasmid # 85494). pET28-UBE1 was a gift from Deschaies lab. pDEST17-Cdc34a was a gift from Wade Harper (Addgene plasmid # 18674). pET28aLIC-UEV1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25619). pETSUMOhUbc13 was a gift from Cynthia Wolberger (Addgene plasmid # 51131). pET16b-PCNA was a gift from Andrew Deans (Addgene plasmid # 134898). ..

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Article Title: Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems.
    Article Snippet: .. BL21(DE3) strain Escherichia coli were transformed with pET28a-sfGFP, a gift from Ryan Mehl (Addgene plasmid #85492). ..

    Generated:

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Synthesized:

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Expressing:

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Sequencing:

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Polymerase Chain Reaction:

    Article Title: Chemigenetic Encoding of Fluorescent Dyes Enables High‐Fidelity and Wash‐Free Imaging of Proteins in Live Cells
    Article Snippet: .. The GLH and CLH constructs were generated using the pET22b-Halo-tag7-His6 (synthesized commercially) prokaryotic expression vector as follows: First, sfGFP sequence with 5 amino acids linker added to the C-terminal was PCR-amplified from pET28a-sfGFP (Addgene #85492). mCherry sequence with 4 amino acids linker added to the C-terminal was PCR-amplified from pCMV-mCherry-Tubulin-6 (Addgene #55147). .. Then, the vector pET22bHalo-tag7-His6 was digested with EcoRI restriction enzyme (TAKARA), the sfGFP or mCherry sequences was connected by One Step Cloning Kit (YEASEN) to obtain pET22bGLH-His6 and pET22b-CLH-His6 plasmids, respectively.

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Cloning:

    Article Title: Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae
    Article Snippet: .. Constructs for producing TtX122A and TtX122B fused with GFP were generated by cloning the sfGFP coding sequence into our existing TtX122A and TtX122B pET26b constructs using the PIPE cloning method (Klock & Lesley, 2009 ). pET28a-sfGFP [a gift from Ryan Mehl, Addgene plasmid 85492 (Peeler & Mehl, 2012 )] was used as a polymerase chain reaction template to generate the GFP insert. ..

    Transformation Assay:

    Article Title: Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems.
    Article Snippet: .. BL21(DE3) strain Escherichia coli were transformed with pET28a-sfGFP, a gift from Ryan Mehl (Addgene plasmid #85492). ..



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    Schematic representation of the genetic constructs of the dual-inducer system ( a ) and two single‑reporter constructs ( b ) for <t>fluorescent</t> reporter proteins expression. c , d Fluorescence fold change of dual‑inducer vs. single‑reporter across inducer concentrations. c mCherry-only vs. mCherry-dual: NS at 0 µM IPTG; P = 0.0003, <0.0001 ( P = 0.00000058), and <0.0001 ( P = 0.000003) at 20, 200, and 400 µM. d <t>sfGFP-only</t> vs. sfGFP-dual: NS for all. e , f Fluorescence fold change of the dual-inducer vs. single-reporter without the corresponding inducer. e mCherry-dual vs. mCherry-only. P = 0.00461, 0.0165, <0.0001 ( P = 0.00002) at 0, 50, 500 ng/mL aTc. NS at 1000 ng/mL. f sfGFP-dual vs. sfGFP-only. NS at 0, 20, 200 µM IPTG; P = 0.00461 at 400 μM. Schematic representation of the dual-inducer system induced with varying concentrations of IPTG at fixed aTc concentration ( g ) or with varying concentrations of aTc at fixed IPTG concentration ( j ). Fold change in mCherry ( h ) and sfGFP ( k ). mCherry: 0 vs. 500 ng/mL aTc across IPTG concentrations: NS at 0, 20 µM; P = 0.0238, 0.0044 at 200, 400 µM. sfGFP: 0 vs. 200 µM IPTG across aTc concentrations: NS at 0, 50 ng/mL; P = 0.0089, 0.0012 at 500, 1000 ng/mL. Fold change in sfGFP ( i ) and mCherry ( l ). i sfGFP-dual cultures at 500 ng/mL aTc without IPTG vs. 0–400 µM IPTG: NS at 0 µM; P = 0.0009, 0.0007, 0.0010 at 20, 200, 400 µM. l mCherry-dual cultures at 200 µM IPTG without aTc vs. 0–1000 ng/mL aTc: NS at 0 ng/mL; P = 0.0478, 0.0013, 0.0145 at 50, 500, 1000 ng/mL. Fold change in c – f , h , i , k , l is plotted in arbitrary units (AU, y -axis), data representing mean ± s.d. ( n = 6 biologically independent samples). Statistics: c , d , h , k by two-tailed unpaired Welch t-test; e , f , i , l vs. grey controls by Brown–Forsythe and Welch one-way ANOVA with Dunnett T3 correction.
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    Image Search Results


    Schematic representation of the genetic constructs of the dual-inducer system ( a ) and two single‑reporter constructs ( b ) for fluorescent reporter proteins expression. c , d Fluorescence fold change of dual‑inducer vs. single‑reporter across inducer concentrations. c mCherry-only vs. mCherry-dual: NS at 0 µM IPTG; P = 0.0003, <0.0001 ( P = 0.00000058), and <0.0001 ( P = 0.000003) at 20, 200, and 400 µM. d sfGFP-only vs. sfGFP-dual: NS for all. e , f Fluorescence fold change of the dual-inducer vs. single-reporter without the corresponding inducer. e mCherry-dual vs. mCherry-only. P = 0.00461, 0.0165, <0.0001 ( P = 0.00002) at 0, 50, 500 ng/mL aTc. NS at 1000 ng/mL. f sfGFP-dual vs. sfGFP-only. NS at 0, 20, 200 µM IPTG; P = 0.00461 at 400 μM. Schematic representation of the dual-inducer system induced with varying concentrations of IPTG at fixed aTc concentration ( g ) or with varying concentrations of aTc at fixed IPTG concentration ( j ). Fold change in mCherry ( h ) and sfGFP ( k ). mCherry: 0 vs. 500 ng/mL aTc across IPTG concentrations: NS at 0, 20 µM; P = 0.0238, 0.0044 at 200, 400 µM. sfGFP: 0 vs. 200 µM IPTG across aTc concentrations: NS at 0, 50 ng/mL; P = 0.0089, 0.0012 at 500, 1000 ng/mL. Fold change in sfGFP ( i ) and mCherry ( l ). i sfGFP-dual cultures at 500 ng/mL aTc without IPTG vs. 0–400 µM IPTG: NS at 0 µM; P = 0.0009, 0.0007, 0.0010 at 20, 200, 400 µM. l mCherry-dual cultures at 200 µM IPTG without aTc vs. 0–1000 ng/mL aTc: NS at 0 ng/mL; P = 0.0478, 0.0013, 0.0145 at 50, 500, 1000 ng/mL. Fold change in c – f , h , i , k , l is plotted in arbitrary units (AU, y -axis), data representing mean ± s.d. ( n = 6 biologically independent samples). Statistics: c , d , h , k by two-tailed unpaired Welch t-test; e , f , i , l vs. grey controls by Brown–Forsythe and Welch one-way ANOVA with Dunnett T3 correction.

    Journal: Nature Communications

    Article Title: Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability

    doi: 10.1038/s41467-025-67667-8

    Figure Lengend Snippet: Schematic representation of the genetic constructs of the dual-inducer system ( a ) and two single‑reporter constructs ( b ) for fluorescent reporter proteins expression. c , d Fluorescence fold change of dual‑inducer vs. single‑reporter across inducer concentrations. c mCherry-only vs. mCherry-dual: NS at 0 µM IPTG; P = 0.0003, <0.0001 ( P = 0.00000058), and <0.0001 ( P = 0.000003) at 20, 200, and 400 µM. d sfGFP-only vs. sfGFP-dual: NS for all. e , f Fluorescence fold change of the dual-inducer vs. single-reporter without the corresponding inducer. e mCherry-dual vs. mCherry-only. P = 0.00461, 0.0165, <0.0001 ( P = 0.00002) at 0, 50, 500 ng/mL aTc. NS at 1000 ng/mL. f sfGFP-dual vs. sfGFP-only. NS at 0, 20, 200 µM IPTG; P = 0.00461 at 400 μM. Schematic representation of the dual-inducer system induced with varying concentrations of IPTG at fixed aTc concentration ( g ) or with varying concentrations of aTc at fixed IPTG concentration ( j ). Fold change in mCherry ( h ) and sfGFP ( k ). mCherry: 0 vs. 500 ng/mL aTc across IPTG concentrations: NS at 0, 20 µM; P = 0.0238, 0.0044 at 200, 400 µM. sfGFP: 0 vs. 200 µM IPTG across aTc concentrations: NS at 0, 50 ng/mL; P = 0.0089, 0.0012 at 500, 1000 ng/mL. Fold change in sfGFP ( i ) and mCherry ( l ). i sfGFP-dual cultures at 500 ng/mL aTc without IPTG vs. 0–400 µM IPTG: NS at 0 µM; P = 0.0009, 0.0007, 0.0010 at 20, 200, 400 µM. l mCherry-dual cultures at 200 µM IPTG without aTc vs. 0–1000 ng/mL aTc: NS at 0 ng/mL; P = 0.0478, 0.0013, 0.0145 at 50, 500, 1000 ng/mL. Fold change in c – f , h , i , k , l is plotted in arbitrary units (AU, y -axis), data representing mean ± s.d. ( n = 6 biologically independent samples). Statistics: c , d , h , k by two-tailed unpaired Welch t-test; e , f , i , l vs. grey controls by Brown–Forsythe and Welch one-way ANOVA with Dunnett T3 correction.

    Article Snippet: The monomeric Cherry red fluorescent protein (mCherry) gene was obtained from Addgene (plasmid #29747), and the superfolder green fluorescent protein (sfGFP) gene was acquired from Addgene (plasmid #85492).

    Techniques: Construct, Expressing, Fluorescence, Concentration Assay, Two Tailed Test